A biology lab report on the properties of the amylase enzyme

Genetic engineering techniques A gene gun uses biolistics to insert DNA into plant tissue. Creating a genetically modified organism GMO is a multi-step process. Genetic engineers must isolated the gene they wish to insert into the host organism. This can be taken from a cell containing the gene [3] or artificially synthesised.

A biology lab report on the properties of the amylase enzyme

Introduction In addition to your textbook, below are some web resources that will add more background information about enzymes: This is relatively easy to understand.

How do these reactions occur? This is not so easy to understand.

Chemical reactions involve the breaking and reforming of chemical bonds between molecules substrate s of the reactionwhich are transformed into different molecules product s of the reaction.

Chemical reactions can occur spontaneously without added energy or interventionand indeed many of the chemical reactions necessary for life processes are spontaneous; some however, are not.

Metabolic pathways are processes, which involve many chemical reactions that occur in a specific order. For example, to get energy out of a molecule of glucose, a series of reactions must take place in a specific order to break the bonds between the carbons of the glucose molecule.

In addition, you have to rely on a series of chemical reactions that break down stored glycogen into glucose molecules to have glucose molecules in the first place. Enzymes catalyze chemical reactions so that they occur in a timely and sequential manner to produce a product.

Enzymes are biological catalysts. They help to increase the rate of chemical reactions. Enzymes are most often proteins and their three-dimensional shape is important to their catalytic activity. Because of their 3-D shape, enzymes are highly specific for the reactions that they catalyze.

In other words, they are highly specific for the substrates that they will act upon. So any one "function", such as getting energy from a glucose molecule, actually involves many reactions, each with a specific enzyme.

Enzyme activity is influenced by many factors. You will be examining some of the major factors, which influence the activity of an enzyme called catalase. Catalase is an enzyme, which is found in many cells, but in highest levels in the liver because the liver often functions to break down toxins present in the blood.

Catalase catalyzes the break down of hydrogen peroxide: Follow this link to get more helpful information about catalase. Each lab station is set up to perform a different section of the Lab exercises A-F. Check with your lab instructor for updated information about which sections your group is responsible for.

The activities are as follows: The protocol below describes how to measure the activity of liver catalase by measuring the amount of O2 that is produced when liver catalase is combined with its substrate, hydrogen peroxide H2O2.

You will collect data in terms of the production of one of the products volume of O2 produced in mLand you will then convert to enzyme activity units by dividing the volume of oxygen produced by the amount of time you allow the experiment to run.

In this lab for ease of calculation, you will measure for one minute. For example, after setting up the apparatus as described below, you will then measure the amount of O2 collected in the graduated cylinder after one minute. If the amount of O2 produced after one minute of reaction time is 32 mL, then the enzyme activity is: For each parts A-F, you will be graphing enzyme activity on the Y-axis the dependent variable and the independent variable parts A-F on the X-axis.

Your instructor will give you clear directions on how to prepare your complete lab report. Protocol Work as a team of 4 at your table to perform the experiment. Each group will be assigned one or more specific experiments in lab.

At your table you will find a small rectangular bottle fitted with a rubber stopper and metal tube reaction vessela ml graduated cylinder and holder, a plastic pan, and a supply of small pieces of filter paper. Submerge the graduated cylinder to fill it with water. Turn the graduated cylinder upside down, keeping the open end under water, and suspend it upside down in the clamp.

Adjust the height of the clamp so the open end of the cylinder is about 2 cm below the surface of the water.

A biology lab report on the properties of the amylase enzyme

Place a thermometer in the pan and sometime during Part A, record the temperature of the water. Figure 1 shows a picture of the setup. Experimental set up for enzyme lab For all parts A through F, each reaction vessels will have 3 soaked disks and 10 ml substrate solutions as shown in Figure 2 below: Reaction vessel The following procedures are used to obtain oxygen production and are to be repeated for all Sections A-F.

Remove the stopper and lay the reaction vessel on its side on the table. Then, using forceps, dip three filter disks one at a time in the enzyme solution.Lab Report: Enzymes - Free download as Word Doc .doc), PDF File .pdf), Text File .txt) or read online for free. How enzymes work is fascinating.

Take a look at this lab report for a better understanding of how enzymes work/5(12). This independent site is for education and information about digestive enzymes.

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Amylase on starch lab -

Quantabio Resource Center. Download the most current documents for your Quantabio product. Use the search bar for a simple keyword search or apply product and material filters to limit search results. Highlights Structural comparison of glucansucrase three-dimensional structures.

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Activity of Enzymes Biology Lab Report - Lab Report